{"product_id":"dmem-cell-culture-medium-low-glucose-500-ml-b0btjrpp3r","title":"DMEM Cell Culture Medium\/Low Glucose","description":"\u003ch2\u003eKey Features of Hygia DMEM Low Glucose\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eProven Formulation:\u003c\/strong\u003e Supports primary fibroblasts, neurones, glial cells, HUVECs, smooth muscle cells, and common lines such as HeLa, 293, Cos-7, and PC-12.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSterile and Ready-to-Uses:\u003c\/strong\u003e Provided as a sterile liquid following 0.1 µm filtration and aseptic filling in a hundred-stage FFU. Filter integrity tests are performed both before-and-after filtration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ecGMP Compliant:\u003c\/strong\u003e Manufactured at a cGMP-compliant facility using water for injection. Each lot is tested for sterility, pH, osmolality, endotoxin, and cell culture performance.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSupplement Required:\u003c\/strong\u003e Contains no proteins, lipids, or growth factors; typically needs 10% foetal bovine serum (FBS) added before uses.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eQuality and Manufacturing Standards\u003c\/h2\u003e\u003cp\u003eHygia Reagents ensures rigorous quality control. All raw materials undergo strict testing. The final product is filtered through 0.1 µm sterile filters, with integrity tests verified. Each lot is subjected to sterility, pH, osmolality, endotoxin, and cell culture testing to guarantee consistent performance.\u003c\/p\u003e\u003ch2\u003eWhat Is DMEM Cell Culture Medium?\u003c\/h2\u003e\u003cp\u003eDMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for cultivating a variety of mammalian cells. It contains four times the concentration of amino acids and vitamins found in Eagle's Minimal Essential Medium, making it a nutrient-rich choice for cell culture. This low glucose formulation (1.0 g\/L) includes sodium pyruvate and uses phenol red as a pH indicator. The medium relies on a sodium bicarbonate buffer system (3.7 g\/L) and requires a 5–10% CO₂ environment to maintain physiological pH.\u003c\/p\u003e\u003ch2\u003eHow to Uses DMEM for Cell Culture\u003c\/h2\u003e\u003cp\u003eThis medium is designed for aseptic cell culture techniques. Warm the medium to 37°C before adding to cell cultures. Supplement with 10% FBS or other required additives. Maintain cultures in a humidified incubator with 5–10% CO₂. Avoid prolonged exposure to light to prevent degradation of phenol red and other light-sensitive components.\u003c\/p\u003e\u003ch2\u003eFAQs About DMEM Cell Culture Medium\u003c\/h2\u003e\u003ch3\u003eWhat cell types can be grown in this DMEM?\u003c\/h3\u003e\u003cp\u003eThis low-glucose DMEM supports primary cells (fibroblasts, neurones, glial cells, HUVECs, smooth muscle cells) and established lines such as HeLa, 293, Cos-7, and PC-12.\u003c\/p\u003e\u003ch3\u003eDo I need to add anything before uses?\u003c\/h3\u003e\u003cp\u003eYes, DMEM requires supplementation, commonly with 10% foetal bovine serum (FBS) and antibiotics if desired. It contains no proteins, lipids, or growth factors.\u003c\/p\u003e\u003ch3\u003eWhy is CO₂ necessary?\u003c\/h3\u003e\u003cp\u003eThe sodium bicarbonate buffer system requires a 5–10% CO₂ atmosphere to maintain the correct pH for cell growth.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research uses only. Not for diagnostic or therapeutic purposes.\u003c\/em\u003e\u003c\/p\u003e","brand":"Hygia Reagents","offers":[{"title":"Default Title","offer_id":46724824170657,"sku":null,"price":35.14,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0740\/0043\/3313\/files\/618XUmaOdSL._SL1500.jpg?v=1784559686","url":"https:\/\/ukvitalityvitamins.co.uk\/products\/dmem-cell-culture-medium-low-glucose-500-ml-b0btjrpp3r","provider":"UkVitalityVitamins","version":"1.0","type":"link"}