DMEM Cell Culture Medium/Low Glucose

Key Features of Hygia DMEM Low Glucose

• Proven Formulation: Supports primary fibroblasts, neurones, glial cells, HUVECs, smooth muscle cells, and common lines such as HeLa, 293, Cos-7, and PC-12.
• Sterile and Ready-to-Uses: Provided as a sterile liquid following 0.1 µm filtration and aseptic filling in a hundred-stage FFU. Filter integrity tests are performed both before-and-after filtration.
• cGMP Compliant: Manufactured at a cGMP-compliant facility using water for injection. Each lot is tested for sterility, pH, osmolality, endotoxin, and cell culture performance.
• Supplement Required: Contains no proteins, lipids, or growth factors; typically needs 10% foetal bovine serum (FBS) added before uses.

Quality and Manufacturing Standards

Hygia Reagents ensures rigorous quality control. All raw materials undergo strict testing. The final product is filtered through 0.1 µm sterile filters, with integrity tests verified. Each lot is subjected to sterility, pH, osmolality, endotoxin, and cell culture testing to guarantee consistent performance.

What Is DMEM Cell Culture Medium?

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for cultivating a variety of mammalian cells. It contains four times the concentration of amino acids and vitamins found in Eagle's Minimal Essential Medium, making it a nutrient-rich choice for cell culture. This low glucose formulation (1.0 g/L) includes sodium pyruvate and uses phenol red as a pH indicator. The medium relies on a sodium bicarbonate buffer system (3.7 g/L) and requires a 5–10% CO₂ environment to maintain physiological pH.

How to Uses DMEM for Cell Culture

This medium is designed for aseptic cell culture techniques. Warm the medium to 37°C before adding to cell cultures. Supplement with 10% FBS or other required additives. Maintain cultures in a humidified incubator with 5–10% CO₂. Avoid prolonged exposure to light to prevent degradation of phenol red and other light-sensitive components.

FAQs About DMEM Cell Culture Medium

What cell types can be grown in this DMEM?

This low-glucose DMEM supports primary cells (fibroblasts, neurones, glial cells, HUVECs, smooth muscle cells) and established lines such as HeLa, 293, Cos-7, and PC-12.

Do I need to add anything before uses?

Yes, DMEM requires supplementation, commonly with 10% foetal bovine serum (FBS) and antibiotics if desired. It contains no proteins, lipids, or growth factors.

Why is CO₂ necessary?

The sodium bicarbonate buffer system requires a 5–10% CO₂ atmosphere to maintain the correct pH for cell growth.

For research uses only. Not for diagnostic or therapeutic purposes.